The micro-zone hardness of titanium alloy matrix of Ti-6Al-4Sn-10Zr-1Mo-1Nb-1W-0.3Si and 3vol%TiBw reinforced high temperature titanium matrix composites were investigated by nano-indentation, respectively. The results show that the average hardness of titanium matrix composites is 6.18 GPa, which is higher than titanium alloy matrix by 14.4%. By comparing the hardness with different thickness of α lamellar and the hardness with different distances to the TiBw respectively, it is found that the TiBw reinforced high temperature titanium matrix composites present higher hardness than the titanium alloy matrix. The reason is that the matrix microstructure is refined by TiBw and the deformation of matrix near the TiBw is easy to be blocked. The hardness data show a large dispersion due to the inhomogeneous microstructure caused by the addition of TiBw.