钛表面处理后表面能分析及对细胞附着的影响

Surface Energy Analysis of Treated Titanium and Effects on Cell Adhesion

摘要:

采用微弧氧化（MAO）及水热合成（MAO-HS）法对纯钛表面进行处理，然后对其表面能进行分析，并观察成骨细胞在其表面的附着动力学变化及骨架蛋白表达。结果显示MAO表面能（50.6mj/m2）。细胞在MAO-HS表面的附着率明显高于MAO及Ti表面。细胞骨架蛋白在3种材料表面均表达良好，但彼此之间存在差异。因此钛有面采用微弧氧化及水热合成法处理后表面形貌及表面能发生改变，这种改变影响了细胞在其表面的生物为行为。

Abstract:

In this study the micro-arc oxidized (MAO) and hydrothermally synthesized (MAO-HS) methods were used to modify the TiO2 layer of titanium. The surface characters and the effects on cell behavior, which included cell adhesion kinetics and cytoskeletal organizations were need to evaluate. The surface energy was examined by physicochemical methods. Biological assays were performed with the mouse osteoblastic cell line MC3T3-E1, cells were inoculated on the treated surface of the materials. The surface energy of MAO (50.6 mJ/m2) and MAO-HS (58.5 mJ/m2) was higher than titanium (39.6 mJ/m2 ). A significantly higher cell adhesion rate was observed on the MAO-HS surface than on the MAO and titanium surface. The cytoskeletal organizations were analyzed by actin and vinculin staining on all the samples after being cultured 2 hours. Modification of TiO2 layer on titanium surface has an influence on the surface characters and the cell behavior on the materials.