纯钛表面PPy／rhBMP-2复合涂层对骨髓间充质干细胞增殖、分化的影响

Effects of PPy/rhBMP-2 Composite Coating of Pure Titanium on Proliferation and Differentiation of Bone Mesenchymal Stem Cells in vitro

摘要:

采用水溶液恒电流电化学聚合法在纯钛表面制备了聚吡咯／重组人骨形成蛋白涂层（Ti／PPy／rhBMP-2）。利用循环伏安扫描分析涂层氧化还原性质，^125I蛋白质标记法检测rhBMP-2掺杂量，表面粗糙度仪、扫描电子显微镜检测涂层微观形貌。通过对骨髓间充质干细胞（BMSCs）在Ti／PPy／rhBMP-2表面增殖、分化能力的检测，评价Ti／PPy／rhBMP-2对骨髓间充质干细胞增殖、分化的影响。结果表明，rhBMP-2能够以聚电解质阴离子方式稳定地掺杂于PPy结构中，并且所生成的涂层表面粗糙度高且结构疏松。Ti／PPy／rhBMP-2中掺杂固定的rhBMP-2依然保持着其骨诱导活性，能够促进BMSCs的增殖、分化。

Abstract:

PPy/rhBMP-2 composite coating on the pure Ti-substrate(Ti/PPy/rhBMP-2)has been successfully electrosynthesized by constant current method in aqueous media.The cyclic voltammetry demonstrated the redox reaction of the composite coating.125I radiolabeling technique was used to measure the amount of rhBMP-2 that was doped into Ti/PPy/rhBMP-2.Goniometer and SEM were used to characterize surface morphology.The effects of Ti/PPy/rhBMP-2 on the proliferation and differentiation of BMSCs were evaluated.The results indicated that rhBMP-2 could be incorporated into Ti/PPy/rhBMP-2 in the stable form of polyelectrolyte anion and the coating showed high roughness and loose structure.The doped rhBMP-2 can still keep the ability of osteoinduction and promote the proliferation and differentiation of BMSCs.