细菌在生物活性钛表面的粘附行为

Adhesion Behavior of Bacteria on Bioactive Titanium Metals

摘要:

以经酸碱处理、碱热处理和阳极氧化处理后的生物活性钛材为研究对象，对材料进行了表面形貌分析和表面能的测定, 并选用常见感染细菌金黄色葡萄球菌和大肠杆菌为研究菌株，采用体外共培养法培养，通过SEM对细菌的形态、在材料表面的粘附情况和粘附量进行观察，通过MTT法对细菌粘附量进行半定量测定，综合评价生物活性钛的表面结构对细菌增殖和粘附的影响。 结果表明，在3种生物活性钛材料表面，细菌的粘附和增殖受材料表面形貌影响显著，其中碱热处理钛表面和阳极氧化钛表面不利于细菌的增殖和粘附，显示出较好的抑菌性能

Abstract:

The titanium metals subjected to surface modification have good biocompatibility, bioactivity and low cell toxicity. It could induce apatite formation in vitro and bone bonding in vivo. However, the infection of implant is a serious problem for the clinic, so it is important to study the interaction of titanium metal with germs involving in the infection. The titanium metals subjected to anodic oxidation treatment (AO-Ti), acid-alkali treatment (AA-Ti) and alkali-heat treatment (AH-Ti) were used in this study. The germs of Gram-positive strain of Staphylococcus aureus (ATCC 25923) and Gram-negative strain of Escherichia coli（ATCC 25922）were cultured on these bioactive metals. The material surfaces were characterized by SEM and DSA. The effects of surface properties on the germs were evaluated by the investigation of the cell extrinsic shape, the proliferation of bacteria according to SEM and MTT observations. The results show that the Ti metals subjected to alkali-heat treatment (AH-Ti) and anodic oxidation treatment (AO-Ti) have good bacterial restrain properties. The titanium surface topography has great effect on the bacterial adhesion and proliferation on its surface