NiTi合金表面微观形貌对血管内皮细胞及血小板黏附的影响

Effects of Micropatterned Surface of NiTi Alloy on Adhesion of Endothelial Cells and Blood Platelets

摘要:

主要探讨血管支架材料——NiTi合金不同表面形貌对牛主动脉血管内皮细胞及血小板黏附的影响。采用机械抛光、机械刻蚀和化学浸蚀的方法制备微孔、微凹槽等微结构特征的NiTi合金表面。利用扫描电镜、粗糙度轮廓仪等对材料表面微观形貌和平均粗糙度进行表征，并测定微孔和微凹槽的材料表面对血小板及血管内皮细胞黏附的影响。结果表明：NiTi合金基体表面制备纳米级粗糙度的微孔和微凹槽等不同微观形貌对血小板黏附的影响不显著，但可明显促进内皮细胞的黏附；具有微孔结构的材料表面黏附的细胞数量最多，且细胞生长状态良好；材料表面微凹槽结构对细胞的早期黏附具有接触诱导效应。微粗糙化的各种不同材料表面形貌对血小板黏附的影响不显著

Abstract:

Effects of the surface morphology of micropatterned NiTi alloy, an intravascular stent material, on the adhesion of bovine aortic endothelial cells (BAECs) and blood platelets were investigated. Mechanical polishing, chemical pickling and sandpaper grinding were used to prepare NiTi alloy with micropores and microgrooves. The surface morphologies and average roughness (Ra) were characterized by SEM and surface profilometer. The effects of the material surface with micropores and microgrooves on the adhesion of blood platelets and endothelial cells were determined. Results show the NiTi alloy surface with micropores or microgrooves could be controlled in the range of nanometer. Both of these two micriopatterned surface could enhance BAECs adhesion, but the effect on platelet adhesion is not obvious; the number of BAECs adhesion on surface with micropores were significantly more than that on the surface with microgrooves. Furthermore, the cells in the surface with micropores show a good growth situation. The microgroove structure has contact-induction effect on the early adhesion of the cells