钛表面自组装硅烷化对仿生矿化性能和细胞增殖的影响

Effect of Silanization on Titanium Surfaces by SAM on Biomimetic Mineralization Properties and Osteoblast Proliferation

摘要:

通过自组装技术以3-氨丙基三乙氧基硅烷为主要成分的有机溶液对纯钛进行表面改性处理，进一步将改性处理后的样品浸泡在模拟体液中仿生矿化处理，利用全反射红外光谱、扫描电镜和X射线衍射研究矿化处理前后样品的表面形貌与结构特征；将人骨髓间充质干细胞（HMSCs）种植于改性处理前后样品表面，利用四甲基偶氮唑盐微量酶反应比色法(MTT法)和扫描电镜观察细胞在改性前后样品表面的黏附、生长和增殖行为。结果表明，仿生矿化处理后，样品表面有类骨磷灰石生成，其钙/磷比为1.3；与纯钛相比，自组装硅烷化处理样品表面HMSCs的黏附率高，增殖效果明显。

Abstract:

The pure titanium plates were modified by SAM using the organic solution with 3-aminopropyltriethoxysilane as the chief component and subsequently exposed to simulated body fluid for biomimetic mineralization. The surface morphologies and structures were characterized by attenuated total refraction-Fourier transform infrared spectroscopy (ATR-FTIR), scanning electronic microscopy (SEM) and X-ray diffraction (XRD); Human sweat gland cells (HMSCs) were planted on the surfaces of samples before and after modification, and then the adhesion and proliferation of HMSCs on the surfaces of titanium and its action before and after modification were investigated by MTT colorimetry and SEM. The results show that HCA coating is synthesized by biomimetic method and the ratio of Ca/P is 1.3. Compared with the pure titanium samples, the adhered HMSCs on the surfaces of titanium after modification by SAM are more, and the proliferation effect is obvious.