生物活性钛体内抑菌性能及其抑菌机理研究

Antibacterial Properties of Bioactive Ti in Vivo and Its Mechanism

摘要:

为研究碱热处理的钛（AH-Ti）的体内抑菌性能及其机理，以纯钛为对照，将其植入大鼠体内，并注入金黄色葡萄球菌液引起感染。通过测定髓过氧化酶活性评价感染程度，用酶联免疫法测定白蛋白和纤维蛋白原的浓度。结果表明，AH-Ti的细菌感染程度较低，白蛋白和纤维蛋白原浓度也较低。本研究证实了AH-Ti在生理环境下具有一定的抑菌性能，其机理为AH-Ti表面选择性吸附白蛋白而阻止了细菌粘附，从而减轻炎症反应，进而使纤维蛋白原的浓度也较低

Abstract:

In order to study the antibacterial properties of bioactive Ti and its mechanism, alkali-heat treated titanium (AH-Ti) was implanted in rat with Staphylococcus aureus suspension to make infection with pure titanium as contrast. The myeloperoxidase activity was detected for evaluating the rat’s inflammation. The concentrations of albumin and fibrinogen were measured by the Enzymemultiplied immunoassay technique. The results show the AH-Ti related inflammation is low along with low albumin and fibrinogen. It is proved AH-Ti has good antibacterial property in vivo. The mechanism for AH-Ti to prevent bacteria adhering to its surface is that its selectively absorbing albumin to its surface weakens the inflammation around the AH-Ti, and the fibrinogen concentration is lower in turn