Doping of Strontium into Natural Bone Powder and Further Cell Experiment

摘要:

优良的骨修复材料可以缩短骨愈合周期，降低不愈合的发生率。锶离子被发现具有促进骨形成的作用，将锶元素掺入骨粉材料将可能制作出一种有前景的骨修复材料。在研究中锶元素被尝试通过离子交换的方法掺入骨粉材料，实验观察了锶元素掺入骨质中的能力。新材料的细胞毒性通过MTT方法进行评价。原代培养的成骨细胞被用来评价新材料的生物相容性。结果发现锶元素在骨粉中的掺入比例可以高达10%，新材料没有细胞毒性，与普通骨粉相比成骨细胞的相对增殖率达到后者的129%，ALP活性则达到后者的132%。因此，通过离子交换方法可以制备性能优异的掺锶同种骨粉移植材料。

Abstract:

Superior bone repairing materials can shorten bone healing periods and lower the incidence of nonunion. Strontium ions are known for enhancing a bone forming effect. Doping of strontium into bone powder will be able to make an attractive bone repairing material. In this study an ion exchange method was tried to dope strontium into bone powder in vitro. The capability to dope strontium into bone tissue was also observed. The cytotoxicity of novel material was investigated by MTT (Methyl thiazolyl tetrazolium assay) method. And further primarily cultured osteoblast was used to evaluate the biocompatibility of novel strontium doped bone sample. It is found that more than 10% exchange ratio of Sr/Ca can be achieved in bone powder. The Sr-doped bone shows no cytotoxicity. Compared with untreated bone samples, osteoblasts show higher proliferation rate (129%) on Sr-doped bone samples. And ALP (Alkaline phosphatase) is 132% higher on Sr-doped bone samples. Thus it is feasible to develop a Sr-doped allograft bone graft by this method.