纯钛材与大变形纯钛材微弧氧化-水热合成复合陶瓷膜层的细胞相容性研究

Cytocompatibility of Composite Ceramic Coating on Titanium and Large Plastic Deformed Titanium by Micro-arc Oxidization-Hydrothermal Synthesis

摘要:

采用微弧氧化-水热法分别在纯钛材及大变形纯钛材表面制备了TiO2/HA复合陶瓷膜层，从细胞毒性试验、细胞增殖实验、细胞黏附实验等方面评价膜层的细胞相容性。结果表明：纯钛材与大变形纯钛材微弧复合陶瓷膜层均无细胞毒性。与纯钛材微弧复合陶瓷膜层相比，大变形纯钛材微弧复合陶瓷膜层表面粗糙度更适宜，结晶形核的HA晶粒的形状及Ca/P比更接近人骨HA，更能有效促进成骨细胞的黏附和铺展，随着培养时间的延长，成骨细胞双层重叠生长结构更为明显。成骨细胞在大变形纯钛材微弧复合陶瓷膜层表面各时间点的吸光度值均更高，细胞相容性更好。

Abstract:

Micro-arc oxidation(MAO) and hydrothermal synthesis(HS) methods were used to prepare hydroxyapatite(HA)and anatase TiO₂ composite ceramic coating on the surface of conventional titanium and large plastic deformed titanium. The cellular compatibility of modified coating was evaluated by cytotoxicity experiment,cell proliferation assay and adhesion behavior of osteoblasts. The results demonstrated that: All samples were found to be noncytotoxic. Compared with the composite ceramic coating of conventional titanium, needle-like HA crystallization, which was closer to the Ca/P ratio of natural human bone, appeared on the surface of composite ceramic coating on large plastic deformed titanium. The composite ceramic coating of large plastic deformed titanium showed smoother and more level surface optimal and could better promote adhesion and spreading of osteoblasts.Optical density of osteoblasts cultured on the composite ceramic coating of large plastic deformed titanium was also significantly higher, and the two-tier growing structure of osteoblasts was more obvious with the progression of cultural time, which implied optimal cytocompatibility.