Preparation and Characterization of Europium-Doped Nanohydroxyapatite (Eu-nHA) Fluorescent Agent

摘要:

采用湿法化学合成含铕纳米羟基磷灰石(Eu-nHA)，并用透射电镜(TEM)、X射线衍射仪(XRD)、元素光谱分析、荧光分光光度计及荧光显微镜成像等进行分析观察。结果表明：稀土元素铕被正确掺入纳米羟基磷灰石晶格内，所制备的含铕纳米羟基磷灰石(Eu-nHA)纳米粒径为5~15 nm (宽) × 15~30 nm (长)。该纳米材料在适宜紫外光激发下能明确产生红色荧光。5D0-7F1和5D0-7F2 跃迁是形成该光致发光的重要原因。本研究制备的Eu-nHA有望成为一种新的纳米荧光剂应用于生物医学领域。

Abstract:

A europium (Eu)-doped nanohydroxyapatite (Eu-nHA) photoluminescent material was synthesized through a wet chemical precipitation method. The prepared Eu-nHA nanomaterial was then characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), spectral analysis of elements, spectrofluorometry and luminescence test. Results show that the Eu-nHA nanoparticles exhibit a tiny rod-like morphology with the particle size of 5~15 nm (width) × 15~30 nm (length). Besides, the nano-crystal pattern of Eu-nHA and successful doping of Eu ions into nHA have also been revealed. Meanwhile, the present study shows that the Eu-nHA agent actually displays functional red fluorescence under proper ultraviolet (UV) irradiation. It is evident that 5D0-7F1 and 5D0-7F2 transition of Eu ions play key roles in the photoluminescence activity. In conclusion, the synthesized Eu-nHA agent holds promise as a novel nano-fluorescence agent for biomedical applications.