Morphology Improvement of Sandblasted and Acid-Etched Titanium Surface and Osteoblast Attachment Promotion by Hydroxyapatite Coating

摘要:

喷砂结合酸洗技术（SLA）和羟基磷灰石（HA）沉积技术是2种广泛用于改善钛种植体表面特征的方法。本研究中，用一种简单的沉积方法将一层HA沉积在SLA处理的钛表面（SLA-Ti）。应用扫描电子显微镜（SEM）和原子力显微镜（AFM）观察SLA-Ti和HA覆盖的SLA-钛（HA-Ti）的表面形貌和测量表面粗糙度。同时对2种钛表面的蛋白质吸附，成骨细胞趋化和成骨细胞附着进行分析。结果表明：SLA-Ti表面布满大量规则的微孔，HA-Ti表面在多微孔的背景下覆盖大量规则的微颗粒。与SLA-Ti相比，HA-Ti表面的粗糙度较低。2种钛表面的蛋白吸附均连续均匀，成骨细胞趋化也无差异。在试件培养12和24 h后，发现HA-Ti表面附着的成骨细胞及成骨细胞间联系比SLA-Ti表面更多。这些研究表明HA涂层改善了SLA-Ti钛的表面形貌和促进了体外成骨细胞附着。

Abstract:

The sandblasting-acid-etched (SLA) technique and hydroxyapatite (HA) deposition are the two methods widely used to improve surface characteristics of titanium implants. In the current study, a layer of HA was deposited on SLA-treated titanium (SLA-Ti) by a simple deposition method. The surface topography and surface roughness of SLA-Ti and HA-coated titanium (HA-Ti) were evaluated using scanning electron microscope (SEM) and atomic force microscope (AFM). Protein adsorption, osteoblast chemotaxis as well as osteoblast attachment on both Ti surfaces were additionally analyzed. The results show that SLA-Ti surface is covered with uniform, multiple micropores, whereas HA-Ti surface is covered with a large number of uniform microparticles under multiple microporous background. In comparison to SLA-Ti, the surface roughness of HA-Ti surface is lower. The protein adsorption on SLA-Ti and HA-Ti surfaces is generally even, and the chemotaxis of osteoblast have no differences. After 12 and 24 h of incubation, more osteoblasts are adhered to the HA-Ti surface and more osteoblast-osteoblast connection is observed on HA-Ti surface compared to SLA-Ti surface. These findings demonstrate that HA coating deposited on SLA-Ti surface improves SLA-Ti surface morphology and promotes osteoblast attachment in vitro.