钛酸盐纳米线控制细胞的粘附及其活性

Investigation of Cell Adhesion and Activity Controlled by Titanate Nanowire

摘要:

控制细胞与生物材料的互动在植入器械包括药物输送系统，生物感应器等方面的应用是非常渴望的。目前我们对于开发能够限制细胞的粘附以及活性的新材料有着较大的期待。在本实验中我们通过水热法在钛片表面制作钛酸盐纳米线支架，研究了钛酸盐纳米线对于MG63成骨细胞粘附、增殖、分化的影响。结果显示与光滑组钛片比较在纳米线表面有较少的细胞粘附，扫描电镜观察可见仅有少量细胞被纳米线穿刺并固定在纳米线表面，并且细胞形态呈不规则的长条形，免疫荧光染色显示细胞不能形成细胞骨架并且细胞形状不规则；随后的细胞增殖也受到了明显的抑制；碱性磷酸酶以及RUNX2活性检测发现纳米线组细胞其分化能力也相应减弱。结论，在纳米线表面细胞早期粘附以及增殖及分化功能受到严重抑制。这种抗细胞粘附的纳米结构在药物缓释系统以及生物感应器等方面有应用的价值。

Abstract:

There’s no dispute that the control of interaction between cell and implant which includes drug delivery system and biosensor is a long-purchased goal. Recently, scientists are looking forward to exploring new materials in restricting cell adhesion and activity. In this essay, titanate tanowire was formed on the surface of titanium disks by hydrothermal route and the interaction between titanate tanowire and MG63 osteoblasts(including cell adhesion, proliferation and differentiation)was investigated. It’s shown that fewer cells were adhered on the smooth titanium disks rather than the nanowire one. Only a few cells were fixed on the tanowire surface which showed an irregular long spindle shape observed by SEM. Immunofluoresense also illustrated an irregular cell shape without forming a cell structure, the proliferation was restricted at the same time. Meanwhile, alkaline phosphatase and RUNX2 activity test shared the same results. It can be well concluded that cell adhesion and activity was seriously restricted titanate nanowire surface, such results can be applied on the further studies of drug delivery system and biosensor.