含稀土铽载锌抗菌硅胶的制备及 协同抗菌机理

Preparation and synergistic antibacterial mechanism of terbium/zincantibacterial silica gel

摘要:

通过溶胶—凝胶法制备出含稀土元素铽的载锌抗菌硅胶，采用单因素实验得到较佳的制备条件：锌离子浓度为0.8 mol·L-1，稀土元素铽浓度为0.003 mol·L-1，反应时间为1 h。选用X射线衍射仪（XRD）、扫描电子显微镜（SEM）、X射线光电子能谱仪（XPS）和电感耦合等离子体发射光谱仪（ICP）等对材料进行表征，结果表明：含稀土铽的抗菌硅胶为无定形态，结构蓬松多孔，锌和铽以氧化物的形态负载于硅胶孔隙中，锌、铽元素含量分别为1.82%和0.073%。抗菌检测结果表明该材料对大肠杆菌的生长速率具有抑制作用，添加稀土铽后材料的抗菌率从72%提高到87%。由协同效应因子（SEF）和协同系数（T/E）判断可知，稀土铽在材料中起到协同抗菌作用。通过材料的比表面积、孔径和锌离子负载量的检测结果，对稀土协同抗菌机理进行初步分析。

Abstract:

Terbium/zinc antibacterial silica gel was prepared by sol-gel method. The better preparation conditions were obtained by controlling single factor variable experiments: The concentration of zinc ion 0.8 mol·L-1，the rare earth terbium concentration 0.003 mol·L-1 and reaction time 1 h. And as-prepared materials were characterized by X-ray diffraction (XRD)，scanning electron microscopy (SEM)，X-ray photoelectron spectroscopy (XPS) andinductive coupled plasma emission spectrometer(ICP) analyses. The results showed that this antibacterial silica gel was amorphous，and its structure was fluffy and porous. The presence of homogeneously mixed zinc and terbium, in the form of ZnO and Tb2O3. The ICP analysis resulted in 1.82% of Zn and 0.073% of Tb Antibacterial test results showed that the material had an inhibitory effect on the growth rate of E.coli. The antibacterial rate of the material after adding rare earth terbium increased from 72% to 87%. Synergistic antibacterial effect of rare earth on materials was judged by synergistic effect factor (SEF) and synergy coefficient (T/E)，terbium was a synergistic antimicrobial agent in materials. The synergistic antimicrobial mechanism of rare earth was preliminarily analyzed through the results of the specific surface area, pore size and the loading capacity of zinc ions.