表面电荷的调控对钛表面纤维连接蛋白吸附能力影响的研究

Regulation of surface charge on titanium surfaces for fibronectin adsorption

摘要:

目的 通过对钛片表面电荷的调控改变纯钛表面的蛋白吸附能力,改善其生物学性能。方法 在钛片表面制作大颗粒喷砂酸蚀的形貌,并分别浸泡在不同的溶液中。分别观察检测不同样本表面形貌及表面元素构成；各组样本的表面粗糙度；以及各组样本表面的电荷状况；最后检测各组样本的纤维连接蛋白吸附能力。结果 各组样本表面具有相似的表面形貌,不同组表面因为浸泡的液体不同其表面元素各不相同；各组样本的表面粗糙度无显著差异；而蛋白吸附能力方面浸泡在氯化钙溶液中的钛片具有最高蛋白吸附能力(P＜0.01),而对照组大颗粒喷砂酸蚀组吸附能力最弱。结论 将钛片浸泡在含有二价钙离子的氯化钙溶液中可以改变钛片表面的电荷状态,提升蛋白吸附能力,从而改善其生物学性能。

Abstract:

Objective The purpose of this study was to investigate the fibronectin adsorptive capacity of pure titanium surfaces and improve their biological properties by regulating their surface charge. Methods The different surfaces of titanium sheet were obtained by sandblasting with large grit and acid- etching (SLA), and then immersing in different solutions. Surface morphology, elemental composition, roughness, and urface charge status were studied, and fibronectin adsorption assay was performed for comparative analysis. Result The surface morphology of each group was similar, but the surface elements of the different groups were different because of immersion in different liquids. There was no significant difference in the surface roughness of each group. Titanium sheets immersed in calcium chloride solution had the highest protein adsorption capacity (P < 0.01), whereas the control group had the lowest adsorption capacity. Conclusions Immersin. Of titanium sheets in calcium chloride solution containing divalent calcium ions can change their surface charge, enhance their protein adsorption capacity, and improve their biological properties.