3D凝胶打印制备用于松质骨工程的TCP多孔支架

Preparation of Porous TCP Scaffolds for Cancellous Bone Engineering by 3D Gel-Printing

摘要:

通过3D凝胶打印（3DGP）技术制备了高强度和高孔隙率的磷酸三钙（TCP）多孔支架，通过扫描电子显微镜（SEM）观察支架的微观形态，并通过初步的动物实验评估了多孔支架的生物相容性。研究结果表明，适合打印的浆料固含量为34%（体积分数），打印支架在长度、宽度和高度方向上的收缩率分别为11.44%±0.20%，9.41%±0.23%和10.57%±0.20%。当支架在1150 ℃下烧结2 h后，支架的抗压强度为22.6±0.12 MPa，孔隙率约为62.1%。初步的动物植入实验显示多孔TCP支架在兔股骨髁缺损处未引起明显的排斥反应，并在骨与支架的连接处未见炎症反应或慢性炎症反应。通过3DGP技术制备的多孔TCP支架具有良好的生物相容性和力学性能，有望满足松质骨的植入要求，为下一步的实验研究打下基础。

Abstract:

High-strength and high-porosity tricalcium phosphate (TCP) scaffolds were prepared by 3D gel-printing (3DGP) technique. The microstructure of the scaffolds was observed by scanning electron microscopy (SEM) and the biocompatibility was evaluated by animal experiments. The results show that the optimal concentration of solid loading of slurry for printing is 34vol%, and the shrinkage of printing scaffolds along length, width, and height directions is 11.44%±0.20%, 9.41%±0.23%, and 10.57%±0.20%, respectively. After the scaffolds are sintered at 1150 °C for 2 h, the compressive strength of the scaffolds is 22.6±0.12 MPa and the porosity is ~62.1%. The animal implantation experiment shows that the porous TCP scaffolds do not cause significant tissue rejection in rabbit femoral condylar defects, and there is no inflammatory reaction or chronic inflammatory reaction at the bone-to-scaffold junction. In conclusion, the porous TCP scaffolds prepared by 3DGP technique have good biocompatibility and excellent strength properties, which are expected to meet the implantation requirements of cancellous bone.