多孔Fe-Mn合金表面微纳形貌调控及其生物相容性评价

Regulation of surface micro-nano structure of porous Fe-Mn alloy and evaluation on its biological performance

摘要:

铁基可降解金属因其良好的生物相容性和优异的机械性能,在骨科植入物领域具有广阔的应用前景,但必须突破其降解速率过慢的瓶颈问题。本研究通过电化学技术对3D打印多孔铁锰合金(Fe-30Mn)支架表面进行去合金化处理。通过扫描电镜观察发现,以盐酸和氯化钠分别作为去合金化处理介质溶液,可以在支架表面形成多微孔网络结构和片状纳米结构。接触角和粗糙度测试显示,两种微纳结构的构建均显著改善了Fe-30Mn支架表面亲水性,并提升了其表面粗糙度,多微孔网络结构更加粗糙并且亲水性更好。利用静态浸泡法和电化学耐腐蚀实验评估合金化处理前后支架的腐蚀速率,发现表面微纳结构的形成可加速Fe-30Mn支架的降解。建立体外成骨细胞培养模型,通过激光共聚焦观察及细胞增殖测试发现,经合金化处理的两种支架均能支撑细胞的贴附和增殖,具有良好的细胞相容性。本研究结果表明,经电化学去合金化处理后,Fe-30Mn支架的降解速度得以增强,同时保持了良好的生物相容性,有望在骨修复领域得到较好应用。

Abstract:

Fe-based biodegradable metals having good biocompatibility and excellent mechanical property exhibit great promise for applications in orthopedic implants, while the slow degradation rate is a major bottleneck. In this study, the surface of the porous Fe-30Mn scaffold was dealloyed by electrochemical technology. When using hydrochloric acid and sodium chloride as medium solution in dealloying treatment, micro-nano porous network and sheet-like structures were formed on the surface of the scaffolds, respectively. The contact angle and surface roughness tests showed that the two micro-nano structure significantly improved the hydrophilicity of the Fe-30Mn scaffold and enhanced its roughness. The Fe-30Mn with micro-nano porous network exhibited higher roughness and hydrophlicity than the Fe-30Mn with sheet-like structures. The static immersion method and electrochemical corrosion test were used to evaluate the degradation rate of the before and after alloyingthe scaffolds. The results showed that the construction of surface micro-nano structure could accelerate the degradation of the scaffold. The in vitro cell culture using MC3T3-E1 cells showed that all porous Fe-30Mn scaffolds had good cytocompatibility. The above results confirmed that the Fe-30Mn scaffold had a suitable degradation rate and good biocompatibility after electrochemical dealloying treatment, suggesting its great clinical application prospect in the fields of bone repair.