阳极氧化铝纳米结构协同当归多糖对乳腺癌细胞生长的影响规律研究

Effect of Anodic Aluminum Oxide Modified by Angelica sinensis Polysaccharide on the Growth of Breast Cancer Cells

摘要:

系统的研究了不同周期的纳米凹坑和纳米柱孔氧化铝膜表面直接修饰当归多糖（ASP）或通过γ-氨丙基三乙氧基硅烷（KH550）共价连接修饰ASP后，乳腺癌细胞MDA-MB-231在其表面生长的细胞形态和活性规律。研究发现，当归多糖直接修饰纳米凹坑和纳米柱孔结构能够有效抑制乳腺癌细胞的活性，对细胞形态影响较小。但纳米凹坑结构的细胞活性抑制效果优于纳米柱孔结构，纳米凹坑在300 nm周期时具有最优的细胞活性抑制效果，抑制率为27.7%。用KH550共价修饰ASP的纳米凹坑和纳米柱孔结构对细胞活性的抑制效果要优于直接修饰ASP时的抑制效果，细胞形态也发生了明显的变化，胞体周围生成大量板状伪足。纳米凹坑结构上的细胞活性抑制效果要优于纳米柱孔结构，纳米凹坑在300 nm周期时抑制效果最佳，抑制率为28.2%。该结果对于药物的设计以及输运具有一定的指导作用。

Abstract:

The surface of nanodent and nanopore structured alumina membranes with different periods were directly modified by Angelica sinensis polysaccharide (ASP) or covalently modified by ASP via γ-aminopropyl triethoxysilane (KH550). After that, the morphology and cell viability of breast cancer cells MDA-MB-231 grew on the modified nanostructured surfaces were systematically studied. It was found that nanodent and nanopore structure directly modified by ASP could effectively inhibit the viability of breast cancer cells, and had little effect on cell morphology. However, the inhibitory effect of nanodent structure on cell viability was superior to that of nanopore structure. Nanodent structure with period of 300 nm had the best inhibitory effect on cell viability, and the inhibitory rate is 27.7%. The nanodent and nanopore structures covalently modified by ASP via KH550 had a better inhibitory effect on cell viability than that directly modified ASP, and the cell morphology changed obviously. A large number of lamellipodia were generated around the cell body. However, the inhibition effect of the nanodent structure on cell viability was better than that of the nanopore structure. Nanodent structure with period of 300 nm had the best inhibitory effect, and the inhibition rate is 28.2%. These results have a certain function for the design and delivery of drugs.