具有载药活性的聚-β-羟基丁酸酯涂层对WE镁合金在模拟体液中的降解行为和细胞功能的影响

Effects of drug-contained Poly-β-hydroxybutyrate coating on degradation behavior of WE magnesium alloys in simulated body fluid and cell function

摘要:

镁及其合金具有作为临床医学中的可吸收生物材料的巨大潜力，但镁合金的腐蚀速率过快，可能导致植入物失效。本研究使用层层自组装技术在WE镁合金表面构建具有药物的聚-β-羟基丁酸酯（PHB）涂层。研究了镁合金与药物涂层在模拟体液（SBF）中的腐蚀行为。通过观察降解过程中溶液浓度和微观结构的变化。从细胞迁移，细胞毒性和细胞凋亡来研究药物涂层对组织细胞的影响。结果表明，具有生物活性药物涂层有效降低了样品在SBF中的腐蚀速率，细胞毒性，凋亡，促进了细胞的迁移。

Abstract:

Magnesium and its alloys have great potential as absorbable biomaterials in clinical medicine, but their rapid corrosion might lead to implant failure. The purpose of this study was to prepare Poly-β-hydroxybutyrate (PHB) coating with drug on the surface of WE magnesium alloys using layer-by-layer self-assembly technique. The corrosion behavior of magnesium alloys with drug coating in simulated body fluid (SBF) was studied. The solution concentration and microstructure change during degradation process were observed. The effects of drug coating on tissue cells were investigated by cell migration, cytotoxicity and apoptosis. The results showed that the bioactive drug coating effectively reduced the corrosion rate of the samples in SBF, cytotoxicity, apoptosis, and promoted the migration of the cells.